ANTIBIOTIC RESISTANCE IN CIMANUK RIVER, GARUT REGENCY, WEST JAVA
Keywords:
E.Coli;, Kontaminasi, Resistensi Antibiotik, Sungai Cimanuk
Abstract
Background: Antibiotic resistance could causing infectious treatment by antimicrobial agent become not effective anymore. This could result in longer care and higher cost for patient. This research aimed to understand antimicrobial resistance from microbe isolated from Cimanuk River, Garut Residence, West Java Province.
Methods: Antibiotic resistance testing in river water was conducted by isolating bacteria into enrichment TSB media and then Blood Agar and also MacConkey Agar by spread plate method. Sample then incubated in 37 °C for 24 hours to gain growing bacterial colony. Colony identification and antibiotic resistance testing was done using BD Phoenix 50.
Results: Result showed that 50% E. coli bacterial isolate from Cimanuk River already developed resistance to Ampicillin, Piperacillin-Tazobactam, and Tetracycline antibiotic.
Conclusion: Antibiotic resistance already occurred in E. coli bacteria isolated from Cimanuk River
References
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Arivo, D. and Dwiningtyas, A. (2019), “Pola kepekaan Escherichia coli penyebab infeksi saluran kemih terhadap antibiotik”, Jurnal Farmasi Malahayati, Vol. 2 No. 1, pp. 12–23.
Belay, W.Y., Getachew, M., Tegegne, B.A., Teffera, Z.H., Dagne, A., Zeleke, T.K., Abebe, R.B., et al. (2024), “Mechanism of antibacterial resistance, strategies and next-generation antimicrobials to contain antimicrobial resistance: a review”, Frontiers in Pharmacology, Vol. 15 No. August, pp. 1–26, doi: 10.3389/fphar.2024.1444781.
CLSI. (2023), Performance Standards for Antimicrobial Susceptibility Testing, M100-ED33 ed., Clinical and Laboratory Standards Institute.
Dickinson, B. (2021), PhoenixTM M50 Automated Microbiology System User’s Manual, BD Diagnostic Systems.
Fachrial, E., Harmileni and Anggraini, S. (2019), Pengantar Teknik Laboratorium Mikrobiologi Dan Pengenalan Bakteri Asam Laktat, Unpri Press.
Google Earth. (2024), “Peta lokasi titik pengambilan sampel Sungai Cimanuk, Kabupaten Garut”, Google.
Hikmah, H., Fadjarajani, S. and Hilman, I. (2021), “Karakteristik Pemukiman Penduduk Di Daerah Aliran Sungai (DAS) Cimanuk Studi Kasus Di Desa Haurpanggung Kecamatan Tarogong Kidul Kabupaten Kabupaten Garut”, Geoducation Journal of Geography Education Universitas Siliwangi, Vol. 2 No. 1, pp. 1–12.
Khakim, L. and Rini, C.S. (2018), “Identifikasi Eschericia coli dan Salmonella sp. pada Air Kolam Renang Candi Pari”, Medicra (Journal of Medical Laboratory Science/Technology), Vol. 1 No. 2, pp. 84–93, doi: 10.21070/medicra.v1i2.1491.
Li, M., Liu, Q., Teng, Y., Ou, L., Xi, Y., Chen, S. and Duan, G. (2019), “The resistance mechanism of escherichia coli induced by ampicillin in laboratory”, Infection and Drug Resistance, Vol. 12, pp. 2853–2863, doi: 10.2147/IDR.S221212.
Mahesh, S., Carmelin, D.S. and Muthusamy, R. (2024), “Bacterial Flora and Treatment Strategies in Women With Escherichia coli Urinary Tract Infections”, Cureus, Vol. 16 No. 3, pp. 1–11, doi: 10.7759/cureus.56552.
Maryani, Monalisa, S.S. and Panjaitan, R.S. (2020), “The Effectiveness of Ketapang Leaves Extract (Terminalia catappa) for Inhabiting the Bacterial Growth of Edwardsiella tarda on In Vitro Test”, Jurnal Perikanan Dan Kelautan, Vol. 10 No. 2, pp. 196–208.
Nurjanah, G.S., Cahyadi, A.I. and Windria, S. (2020), “Escherichia Coli Resistance To Various Kinds of Antibiotics in Animals and Humans: a Literature Study”, Indonesia Medicus Veterinus, Vol. 9 No. 6, pp. 970–983, doi: 10.19087/imv.2020.9.6.970.
Paula, A., Saldanha, P. and Ismawatie, E. (2024), “Prevalensi Escherichia Coli Pada Penderita Infeksi Saluran Kemih di RS Guido Valaderes Tahun 2021-2022”, PLENARY HEALTH : JURNAL KESEHATAN PARIPURNA, Vol. 1 No. 3, pp. 400–405.
Rahman, A. (2017), “Analisis Aliran Pada Daerah Aliran Sungai Cimanuk Hulu (Studi Kasus Cimanuk-Bojongloa Garut)”, Jurnal Konstruksi, Vol. 14 No. 1, pp. 91–100, doi: 10.33364/konstruksi/v.14-1.391.
Reddy, S., Kaur, K., Barathe, P., Shriram, V., Govarthanan, M. and Kumar, V. (2022), “Antimicrobial resistance in urban river ecosystems”, Microbiological Research, Elsevier GmbH, Vol. 263, pp. 1–12, doi: 10.1016/j.micres.2022.127135.
Rismawati, D. (2022), “Cara Pemusnahan Obat yang Rusak/Kadaluwarsa di Rumah Tangga”, Yankes.Kemkes.Go.Id, available at: https://yankes.kemkes.go.id/view_artikel/1972/cara-pemusnahan-obat-yang-rusakkadaluwarsa-di-rumah-tangga (accessed 7 December 2024).
Samreen, Ahmad, I., Malak, H.A. and Abulreesh, H.H. (2021), “Environmental antimicrobial resistance and its drivers: a potential threat to public health”, Journal of Global Antimicrobial Resistance, Elsevier Ltd, Vol. 27, pp. 101–111, doi: 10.1016/j.jgar.2021.08.001.
Suprapto, H., Sudarno and Tito, I.M. (2016), “ISOLASI DAN IDENTIFIKASI BAKTERI KITINOLITIK YANG TERDAPAT PADA CANGKANG LOBSTER AIR TAWAR (Cherax quadricarinatus)”, Jurnal Ilmiah Perikanan Dan Kelautan, Vol. 8 No. 1, pp. 16–25.
Suzuki, Y., Hashimoto, R., Xie, H., Nishimura, E., Nishiyama, M., Nukazawa, K. and Ishii, S. (2019), “Growth and antibiotic resistance acquisition of Escherichia coli in a river that receives treated sewage effluent”, Science of the Total Environment, Elsevier B.V., Vol. 690, pp. 696–704, doi: 10.1016/j.scitotenv.2019.07.050.
WHO. (2024), “Kematian Akibat AMR Diperkirakan Capai 10 Juta Orang pada 2050, Kemenkes dan WHO Launching Strategi Nasional”, World Health Organization, available at: https://www.who.int/indonesia/id/news/detail/20-08-2024-deaths-due-to-amr-estimated-to-reach-10-million-people-by-2050--ministry-of-health-and-who-launch-national-strategy (accessed 7 December 2024).
Windria, S., Cahyaningtyas, A.A., Cahyadi, A.I., Wiraswati, H.L. and Ramadhanti, J. (2023), “Identifikasi Fenotip dan Genotip Staphylococcus aureus Isolat Asal Susu Sapi Perah Mastitis Subklinis di Wilayah Pamulihan, Kabupaten Sumedang Jawa Barat”, Jurnal Sain Veteriner, Vol. 41 No. 2, pp. 215–225, doi: 10.22146/jsv.76052.
Zimbro, M.J., Power, D.A., Miller, S.M., Wilson, G.E. and Johnson, J.A. (2009), Difco & BBL Manual: Manual of Microbiological Culture Media, Citeseer, doi: 10.1002/1521-3773(20010316)40:6<9823::AID-ANIE9823>3.3.CO;2-C.
Published
2024-12-20
How to Cite
1.
Syamsudin R, Sudjana S, Nuari D. ANTIBIOTIC RESISTANCE IN CIMANUK RIVER, GARUT REGENCY, WEST JAVA. JKPharm [Internet]. 20Dec.2024 [cited 30Dec.2024];6(2):68 -76. Available from: https://jurnal.poltekkespalembang.ac.id/index.php/Jkpharm/article/view/2633
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