Difference of Regulatory T Cells, Interleukin 10, Interleukin 6, Interferon (IFN)Γ, and Indoleamin Dioksigenase (IDO) Levels in Women With High and Low ASA A Research Article

  • Nani Sari Murni University of Indonesia
  • Indra Gusti Mansur University of Indonesia
Keywords: ASA, Tregs, IL10, IL6, IFNγ, IDO


Unexplained infertility can be caused by various factors, including genetic, immunological, and idiopathic. One of the immunological factors that play a role in unexplained infertility is antisperm antibodies (ASA). About 10-30% of infertile couples are caused by ASA. This study wanted to analyze differences in cellular specific immune components namely regulator T cells and humoral immune components (cytokines IL10, IL6, IFNγ, and IDO) in women with high ASA and low ASA. Samples with high and low ASA were examined ASA titres using the husband's sperm auto-agglutination test (HSAaT) method. Each group of 6 samples were analyzed, so that the total is 12 samples. Tregs were evaluated using flow cytometry with the human forkhead box P3 (FoxP3) staining kit of Biotech and Device. Serum IL10, IL6, and IFNγ was determined using an Abcam ELISA kit. Serum IDO was determined using an RnD ELISA kit. The data were analysed
using the Mann-whitney tests. There are differences in the Tregs population (p = 0.004), IL10 (p = 0.002), IFNγ (p = 0.002) and IDO (p = 0.041) but there is no difference IL6 levels (p 0.240) in women with high and low ASA. High ASA affects of the Tregs population, IL10, IFNγ, and IDO but has no effect on IL6 cytokines