Plasma Absorbance As The Baseline A Simple Method For Correcting The Results Of Blood Glucose Level On Hemolyzed Specimens

James Perdinan S; Siti Sakdiah; Tamrin Tamrin; Dyah Eriska Pratiwi

James Perdinan S Medical Laboratory Technology Department Health Polytechnic of Ministry of Health in Jambi
Siti Sakdiah Medical Laboratory Technology Department Health Polytechnic of Ministry of Health in Jambi
Tamrin Tamrin Medical Laboratory Technology Department Health Polytechnic of Ministry of Health in Jambi
Dyah Eriska Pratiwi Medical Laboratory Technology Department Health Polytechnic of Ministry of Health in Jambi

Keywords: hemolyzed specimens, correction, absorbance

Abstract

Hemolysis on laboratory specimens can be caused by many factors, in vivo, and in vitro. Increased levels of free hemoglobin in the serum specimen can influence the measurement results of the various test. Hemolysis is often difficult to detect properly. Even for serum specimens that appear to be hemolyze, the laboratory does not have a good protocol to avoid errors. This study aims to develop a simple approach that can be carried out in correcting the measured results of laboratory assays. A correction by calculating the plasma absorbance was applied to blood glucose results from hemolyzed specimens. The correction results were then compared with the levels from the same specimens that not hemolyzed. This study was carried out using the experimental method on blood specimens collected from 15 volunteers. Specimens were treated mechanically to produce some specific hemolysates by forced suppressing the blood through a syringe with 23G needle, in 0 to 6 times. In addition to measuring glucose levels, absorbance measurements were also carried out for each specimen diluted in a buffer solution to calculate the increase in light absorbance as the plasma absorbance. All measured data were analyzed using the Repeated Measure ANOVA. In
this study, the increase in average levels was occurred according to the frequency of treatment given. The differences in the increasing results of each treatment
before being corrected were statistically significant (p <0.01). However, after the measurement baseline was corrected by plasma absorbance, then the difference in all results turned out to be insignificant. (p > 0.05). It can be concluded that the increase in light absorption due to free hemoglobin concentration is the cause of increased results from each hemolysate. However, the measurement of plasma absorbance has been proven as an appropriate procedure for correcting the result of glucose level from hemolyzed specimens before being reported.
glucose