Determining Estimation Factor For Platelet Count Using Microscope With Field Number (Fn) 18

Abstract

The estimation number of platelets has been determined based on the method of Barbara Brown (1976), namely multiplying the average platelet count per field of view of 100x objective enlargement with a number of 20,000/mm 3 . Modern microscope ocular lenses show a wide field of view, so the platelets will clearly be visible. The platelet count estimation factor will be more precise if it is determined based on the Field Number (FN) of the microscope. This study used EDTA blood samples to count the platelet counts using the Sysmex XS800i automatic cell counter, followed by making a blood smear and Wright's stain. To determine the mean platelet rate per objective field of view of 100x in zone V using an Olympus CX21 microscope. Furthermore, the ratio of platelet numbers and the average number of platelets per field of view was determined for each sample. The estimation factor is the total ratio obtained divided by the sample size. The number of samples is 64 samples. The estimation factor for the number of platelets obtained is 22, meaning that 1 platelet per field of view is equivalent to 22 x 109/L. Platelet count estimation can be used for reporting platelet count results if there is no equipment available for platelet count with the estimation factor
according to the FN microscope.