The COMPARISON OF THE QUALITY OF MALARIA PREPARATIONS FLOODED WITH SOAKED IN GIEMSA SOLUTION

Abstract

: Malaria is an infectious disease caused of Plasmodium. Microscopic diagnosis by examining thick and thin blood smears stained with Giemsa is that still the "gold standard". Giemsa is generally given by immersing entire blood stock as in the procedure for making malaria preparations and Giemsa has given by soaking in Giemsa paint as in the procedure making cytology preparations. The aim of this study to determine the quality of malaria preparations stained using the technique of being soaked in Giemsa solution to compare of malaria preparations stained using the technique of being soaked in Giemsa solution. The type of research used was a quasi-experiment with a static group comparison design, 80 preparations were made of 40 blood samples containing Plasmodium which were stained using the flooding technique and soaked in Giemsa solution. From the research carried out, the results of staining using the immersion technique were 35 preparations of good quality and 5 preparations of poor quality and staining using the immersion technique contained 48 preparations of good quality and 2 preparations of poor quality. The results of statistical tests using the Mc Nemar test obtained a sig value. of 0.453 (> 0.05). The result of this research can be concluded that there is no significant difference between preparations stained using the flooding technique and immersion in Giemsa solution.