UJI AKTIVITAS ANTIBAKTERI EKSTRAK BUAH RAMBUSA (Passiflora foetida L.) TERHADAP PERTUMBUHAN BAKTERI Lactobacillus acidophilus ATCC 4356
Keywords:
anti bakteri, cavity cleanser, karies, Lactobacillus acidophilus, rambusa (Passiflora foetida L.)
Abstract
Latar Belakang: Lactobacillus acidophilus adalah bakteri gram-positif yang bersifat anaerobik fakultatif, asidurik, dan asidogenik yang berperan dalam perkembangan karies sekunder. Karies sekunder dapat terjadi setelah gigi direstorasi karena adanya bakteri yang tertinggal di smear layer saat melakukan preparasi kavitas. Karies sekunder dapat dicegah dengan penggunaan cavity cleanser. Buah rambusa mengandung senyawa antibakteri seperti alkaloid, saponin, tanin, triterpenoid, steroid, dan flavonoid yang berpotensi sebagai cavity cleanser.
Metode: Penelitian ini merupakan penelitian eksperimental laboratoris dengan desain penelitian post-test only control group design. Kelompok perlakuan dalam penelitian ini adalah ekstrak buah rambusa dengan konsentrasi 12,5%, 25%, 37,5%, dan 50% serta DMSO 10% sebagai kontrol negatif. Uji daya hambat dilakukan dengan metode difusi cakram Kirby-Bauer dengan media MRS-A. Analisis data hasil penelitian dilakukan dengan menggunakan uji Kruskal Wallis dan dilanjutkan dengan uji Post Hoc Mann-Whitney.
Hasil: Rata-rata diameter zona hambat terbesar adalah 5,30 ± 0,83 mm yang dibentuk oleh ekstrak buah rambusa konsentrasi 50%, sedangkan rata-rata diameter zona hambat terkecil dibentuk oleh ekstrak konsentrasi 12,5% dan kontrol negatif sebesar 0 mm. Hasil uji Kruskal Wallis menunjukkan nilai p < 0,05 artinya terdapat perbedaan signifikan antarkelompok perlakuan dan kontrol negatif.
Kesimpulan: Terdapat aktivitas daya hambat ekstrak buah rambusa (Passiflora foetida L.) terhadap pertumbuhan bakteri Lactobacillus acidophilus ATCC 4356 dengan zona hambat terbesar dibentuk oleh ekstrak buah rambusa konsentrasi 50%.
Kata kunci : anti bakteri; cavity cleanser; karies; Lactobacillus acidophilus; rambusa (Passiflora foetida L.)
References
1. Wen ZT, Huang X, Ellepola K, Liao S, Li Y. Lactobacilli and human dental caries: more than mechanical retention. Microbiology (United Kingdom). 2022;168(6):1–11. https://doi.org/10.1099/mic.0.001196
2. Kunarti S, Sukaton S, Nathania N. The Number Of Lactobacillus acidophilus After Using Chlorhexidine 2%, Laser Diode (405 nm), And Combination Of Chlorhexidine 2% With Laser Diode (405 nm). Conserv Dent J. 2020;9(2):77. https://doi.org/10.20473/cdj.v9i2.2019.77-81
3. Ahirwar SS, Gupta MK, Snehi SK, Kalmegh SD. Dental Caries and Lactobacillus: Role and Ecology in the Oral Cavity. Int J Pharm Sci Res 4818 IJPSR]. 2019;10(11):4818–29.
4. Zhou X, Li Y. Atlas of Oral Microbiology: From Healthy Microflora to Disease. Atlas of Oral Microbiology: From Healthy Microflora to Disease second edition. 2020. 1–107 p. https://doi.org/10.1007/978-981-15-7899-1
5. Bilqis NM, Erlita I, Kania DTP. Uji Daya Hambat Ekstrak Bawang Dayak (Eleutherine palmifolia (L.) Merr.) terhadap Pertumbuhan Bakteri Lactobacillus acidophilus. Dentin J Kedokt Gigi. 2018;II(1):26–31.
6. Rahmasari IR, Firdaus Iwak, Dewi RK. Inhibitory Activity Of Ulin Bark (Eusideroxylon zwageri) Extract to Lactobacillus acidophilus. Dentino J Kedokteran Gigi. 2021;6(2):117. https://doi.org/10.20527/dentino.v6i2.11989
7. Yuanita T, Wahjuningrum DA, Selvia M. The Difference of Antibacterial Power Between Cocoa Peel (Theobroma cacao) Extract 6% Compared to NaOCL 5% Againts Lactobacillus acidophilus. Conserv Dent J. 2021;11(2):67. https://doi.org/10.20473/cdj.v11i2.2021.67-71
8. Siwinata M, Zubaidah N, Soetojo A. The effectivity of cavity cleanser chlorhexidinegluconate 2% and saponin 0.78% of mangoosteen peel. Conserv Dent J. 2020;10(1):19. https://doi.org/10.20473/cdj.v10i1.2020.19-22
9. Krisnadita A, Lestari ES, Setyawan A, Antari AL. Antibacterial Effectiveness Test of Potato Peel Ethanol Extract (Solanum tuberosum L.) against Lactobacillus acidophilus: An In Vitro Study. Majalah Obat Tradisional. 2023;28(2):86–92. https://doi.org/10.22146/mot.81476
10. Olla G, Hasan T, Rupidara AD. Effectiveness test of rambusa (Passiflora foetida L.) fruit extract as a liquid anti-mosquito on the development vector of malaria mosquito (Anopheles sp.). Jambura Edu Biosf J. 2020;2(2):44–50.
11. Han X, Song Y, Huang R, Zhu M, Li M, Requena T, et al. Anti-Inflammatory and Gut Microbiota Modulation Potentials of Flavonoids Extracted from Passiflora foetida Fruits. Foods. 2023;12(15):1–11. https://doi.org/10.3390/foods12152889
12. Aulia R, Sutoro IS. Secondary Metabolite of Rambusa Fruit (Passiflora feotida L) As Antibacterial of Plants. AGRICA J Sustain Dryl Agric. 2023;16(2):192–9. https://doi.org/10.37478/agr.v16i2.3114
13. Wardhani RA, Pardede A. Analysis of Phytochemicals and Antioxidant Activities of Methanol Extracts in Stem, Leaves, Rind, and Fruit of Kelubut Plants (Passiflora foetida). Vol. 5, Jurnal Pendidikan Kimia dan Ilmu Kimia. 2022. https://doi.org/10.31602/dl.v5i2.9343
14. Dewi STR, Afsari Y. Uji Aktivitas Ekstrak Buah Rambusa (Passiflora foetida L.) Terhadap Kerusakan Gigi Penyebab Bakteri Streptococcusmutans. Media Farm. 2017;13(no.2):92–6.
15. Budianto NEW, Budiono NDP. Analisis Pengaruh Pemberian Ekstrak Etanol Buah Rambusa (Passiflora foetida L.) Terhadap Zona Hambat Pertumbuhan Bakteri Escherichia coli. J Herbal, Clin Pharm Sci [Internet]. 2023 Oct 24;5(01:48. https://doi.org/10.30587/herclips.v5i01.6205
16. Karmila, Nuryanti S. Analysis of Vitamin C in Rambusa Fruit (Passiflora foetida L.). Media Eksakta. 2021;17(1):46–51. https://doi.org/10.22487/me.v17i1.819
17. Amboupe DS, Mustaqim WA. Ethnobotany of the Mountain Regions of Southeast Asia : Pandanus Conoideus Lam. [Internet]. Bussmann RW, Paniagua-Zambrana NY, editors. Springer Reference; 2021. 799–806 p.
18. George M. Qualitative & Quantitative Phytochemical analysis on the Leaves & fruits of Passiflora foetida. Int J Pharm Sci Invent [Internet]. 2017;6(3):26–30.
19. Shamsudin NF, Ahmed QU, Mahmood S, Shah SAA, Khatib A, Mukhtar S, et al. Antibacterial Effects of Flavonoids and Their Structure-Activity Relationship Study: A Comparative Interpretation. Molecules. 2022;27(4). https://doi.org/10.3390/molecules27041149
20. Muharni, Fitrya, Farida S. Uji Aktivitas Antibakteri Ekstrak Etanol Tanaman Obat Suku Musi di Kabupaten Musi Banyuasin, Sumatera Selatan Antibacterial Assay of Ethanolic Extract Musi Tribe Medicinal Plant. J Kefarmasian Indonesia.2017;7(2):127–35. https://doi.org/10.22435/jki.v7i2.6070.127-135
21. Rini AA, Supriatno, Rahmatan H. Skrining Fitokimia dan Uji Antibakteri Ekstrak Etanol Buah Kawista (Limonia Acidissima L.) dari Daerah Kabupaten Aceh Besar terhadap Bakteri Escherichia Coli. J Ilmu Keguruan dan Ilmu Pendidikan Unsyiah. 2017;2(1):1–12.
22. Sapara Tu, Waworuntu O. Efektivitas Antibakteri Ekstrak Daun Pacar Air (Impatiens balsamina L.) Terhadap Pertumbuhan Porphyromonas gingivalis. 2016;5(4):10–7. https://doi.org/10.35790/eg.4.2.2016.14161
23. Anggraini W, Nisa SC, DA RR, ZA BM. Aktivitas Antibakteri Ekstrak Etanol 96% Buah Blewah (Cucumis melo L. var. cantalupensis) terhadap pertumbuhan bakteri Escherichia coli. Pharm J Indonesia. 2019;5(1):61–6.
24. Tilarso DP, Muadifah A, Handaru W, Pratiwi PI, Khusna ML. Aktivitas Antibakteri Kombinasi Ekstrak Daun Sirih Dan Belimbing Wuluh Dengan Metode Hidroekstraksi. Chempublish J. 2021;6(2):63–74.
25. Wijaya I. Potensi Daun Alpukat Sebagai Antibakteri. Jurnal Ilmu Kesehatan Sandi Husada. 2020;12(2):695–701. https://doi.org/10.35816/jiskh.v12i2.381
26. Faradina AS, Mastra N, Karta IW. Uji Aktivitas Antibakteri Ekstrak Etanol Akar Encok (Plumbago zeylanica L.) Terhadap Pertumbuhan Pseudomonas aeruginosa Secar In Vitro. Meditory. 2019;7(2):110–8.
27. Meilaningrum AN, Putri NEK, Sastyarina Y. Uji Aktivitas Antibakteri Ekstrak Kombinasi Umbi Bawang Tiwai dan Kulit Buah Lemon Terhadap Pertumbuhan Staphylococcus aureus dan Escherichia coli. Proceeding Mulawarmn Pharm Conf.2021;13(April 2021):8–13. https://doi.org/10.25026/mpc.v13i1.436
28. Daud NS, Arni DP, Idris SA, Saehu MS. Uji Aktivitas Antibakteri Ekstrak Batang Meistera chinensis Terhadap Escherichia coli ATCC 35218.War Farm. 2023;12(1):8–18. https://doi.org/10.46356/wfarmasi.v12i1.236
29. Lianah W, Ayuwardani N, Hariningsih Y. Aktivitas Antibakteri Ekstrak Etanol Seledri (Apium graveolens L) Terhadap Pertumbuhan Bakteri Actinomyces sp. dan Lactobacillus acidophilus. Duta Pharma J. 2021;1(1):32–9.
30. Sakul G, Simbala HEI, Rundengan G. Uji Daya Hambat Ekstrak Etanol Daun Pangi (Pangium edule Reinw. ex Blume) terhadap bakteri Staphylococcus aureus, Escherichia coli dan Pseudomonas aeruginosa. Pharmacon. 2020;9(2):275.
31. Wahyuni R, Guswandi, Rivai H. Pengaruh Cara Pengeringan Dengan Oven, Kering Angin dan Cahaya Matahari Langsung Terhadap Mutu Simplisia Herba Sambiloto. Fak Farm Univ Andalas Sekol Tinggi Ilmu Farm Padang. 2014;6(2):126–33.
32. Mulyono LM. Aktivitas antibakteri ekstrak etanol biji buah pepaya (Carica papaya L.) terhadap Escherichia Coli dan Staphylococcus aureus. Calyptra J Ilm Mhs Univ Surabaya [Internet]. 2013;2(2):1–9.
33. Busman B, Edrizal E, Utami DWP. Uji Efektivitas Ekstrak Buah Anggur Hijau (Vitis Vinivera L.) Terhadap Daya Hambat Laju Pertumbuhan Bakteri Streptococcus Mutans Dan Lactobacillus Acidophilus. Ensiklopedia Sos Rev. 2021;2(3):325–32.
34. Tortora G, Funke B, Case C. Microbiology : Make the Connection Between Lecture , Lab , and the Real World. 2013. Chapter 8.
35. Risna YK, Sri-Harimurti SH, Wihandoyo W, Widodo W. Kurva Pertumbuhan Isolat Bakteri Asam Laktat dari Saluran Pencernaan Itik Lokal Asal Aceh. J Peternak Indones (Indonesian J Anim Sci. 2022;24(1):1. https://doi.org/10.25077/jpi.24.1.1-7.2022
2. Kunarti S, Sukaton S, Nathania N. The Number Of Lactobacillus acidophilus After Using Chlorhexidine 2%, Laser Diode (405 nm), And Combination Of Chlorhexidine 2% With Laser Diode (405 nm). Conserv Dent J. 2020;9(2):77. https://doi.org/10.20473/cdj.v9i2.2019.77-81
3. Ahirwar SS, Gupta MK, Snehi SK, Kalmegh SD. Dental Caries and Lactobacillus: Role and Ecology in the Oral Cavity. Int J Pharm Sci Res 4818 IJPSR]. 2019;10(11):4818–29.
4. Zhou X, Li Y. Atlas of Oral Microbiology: From Healthy Microflora to Disease. Atlas of Oral Microbiology: From Healthy Microflora to Disease second edition. 2020. 1–107 p. https://doi.org/10.1007/978-981-15-7899-1
5. Bilqis NM, Erlita I, Kania DTP. Uji Daya Hambat Ekstrak Bawang Dayak (Eleutherine palmifolia (L.) Merr.) terhadap Pertumbuhan Bakteri Lactobacillus acidophilus. Dentin J Kedokt Gigi. 2018;II(1):26–31.
6. Rahmasari IR, Firdaus Iwak, Dewi RK. Inhibitory Activity Of Ulin Bark (Eusideroxylon zwageri) Extract to Lactobacillus acidophilus. Dentino J Kedokteran Gigi. 2021;6(2):117. https://doi.org/10.20527/dentino.v6i2.11989
7. Yuanita T, Wahjuningrum DA, Selvia M. The Difference of Antibacterial Power Between Cocoa Peel (Theobroma cacao) Extract 6% Compared to NaOCL 5% Againts Lactobacillus acidophilus. Conserv Dent J. 2021;11(2):67. https://doi.org/10.20473/cdj.v11i2.2021.67-71
8. Siwinata M, Zubaidah N, Soetojo A. The effectivity of cavity cleanser chlorhexidinegluconate 2% and saponin 0.78% of mangoosteen peel. Conserv Dent J. 2020;10(1):19. https://doi.org/10.20473/cdj.v10i1.2020.19-22
9. Krisnadita A, Lestari ES, Setyawan A, Antari AL. Antibacterial Effectiveness Test of Potato Peel Ethanol Extract (Solanum tuberosum L.) against Lactobacillus acidophilus: An In Vitro Study. Majalah Obat Tradisional. 2023;28(2):86–92. https://doi.org/10.22146/mot.81476
10. Olla G, Hasan T, Rupidara AD. Effectiveness test of rambusa (Passiflora foetida L.) fruit extract as a liquid anti-mosquito on the development vector of malaria mosquito (Anopheles sp.). Jambura Edu Biosf J. 2020;2(2):44–50.
11. Han X, Song Y, Huang R, Zhu M, Li M, Requena T, et al. Anti-Inflammatory and Gut Microbiota Modulation Potentials of Flavonoids Extracted from Passiflora foetida Fruits. Foods. 2023;12(15):1–11. https://doi.org/10.3390/foods12152889
12. Aulia R, Sutoro IS. Secondary Metabolite of Rambusa Fruit (Passiflora feotida L) As Antibacterial of Plants. AGRICA J Sustain Dryl Agric. 2023;16(2):192–9. https://doi.org/10.37478/agr.v16i2.3114
13. Wardhani RA, Pardede A. Analysis of Phytochemicals and Antioxidant Activities of Methanol Extracts in Stem, Leaves, Rind, and Fruit of Kelubut Plants (Passiflora foetida). Vol. 5, Jurnal Pendidikan Kimia dan Ilmu Kimia. 2022. https://doi.org/10.31602/dl.v5i2.9343
14. Dewi STR, Afsari Y. Uji Aktivitas Ekstrak Buah Rambusa (Passiflora foetida L.) Terhadap Kerusakan Gigi Penyebab Bakteri Streptococcusmutans. Media Farm. 2017;13(no.2):92–6.
15. Budianto NEW, Budiono NDP. Analisis Pengaruh Pemberian Ekstrak Etanol Buah Rambusa (Passiflora foetida L.) Terhadap Zona Hambat Pertumbuhan Bakteri Escherichia coli. J Herbal, Clin Pharm Sci [Internet]. 2023 Oct 24;5(01:48. https://doi.org/10.30587/herclips.v5i01.6205
16. Karmila, Nuryanti S. Analysis of Vitamin C in Rambusa Fruit (Passiflora foetida L.). Media Eksakta. 2021;17(1):46–51. https://doi.org/10.22487/me.v17i1.819
17. Amboupe DS, Mustaqim WA. Ethnobotany of the Mountain Regions of Southeast Asia : Pandanus Conoideus Lam. [Internet]. Bussmann RW, Paniagua-Zambrana NY, editors. Springer Reference; 2021. 799–806 p.
18. George M. Qualitative & Quantitative Phytochemical analysis on the Leaves & fruits of Passiflora foetida. Int J Pharm Sci Invent [Internet]. 2017;6(3):26–30.
19. Shamsudin NF, Ahmed QU, Mahmood S, Shah SAA, Khatib A, Mukhtar S, et al. Antibacterial Effects of Flavonoids and Their Structure-Activity Relationship Study: A Comparative Interpretation. Molecules. 2022;27(4). https://doi.org/10.3390/molecules27041149
20. Muharni, Fitrya, Farida S. Uji Aktivitas Antibakteri Ekstrak Etanol Tanaman Obat Suku Musi di Kabupaten Musi Banyuasin, Sumatera Selatan Antibacterial Assay of Ethanolic Extract Musi Tribe Medicinal Plant. J Kefarmasian Indonesia.2017;7(2):127–35. https://doi.org/10.22435/jki.v7i2.6070.127-135
21. Rini AA, Supriatno, Rahmatan H. Skrining Fitokimia dan Uji Antibakteri Ekstrak Etanol Buah Kawista (Limonia Acidissima L.) dari Daerah Kabupaten Aceh Besar terhadap Bakteri Escherichia Coli. J Ilmu Keguruan dan Ilmu Pendidikan Unsyiah. 2017;2(1):1–12.
22. Sapara Tu, Waworuntu O. Efektivitas Antibakteri Ekstrak Daun Pacar Air (Impatiens balsamina L.) Terhadap Pertumbuhan Porphyromonas gingivalis. 2016;5(4):10–7. https://doi.org/10.35790/eg.4.2.2016.14161
23. Anggraini W, Nisa SC, DA RR, ZA BM. Aktivitas Antibakteri Ekstrak Etanol 96% Buah Blewah (Cucumis melo L. var. cantalupensis) terhadap pertumbuhan bakteri Escherichia coli. Pharm J Indonesia. 2019;5(1):61–6.
24. Tilarso DP, Muadifah A, Handaru W, Pratiwi PI, Khusna ML. Aktivitas Antibakteri Kombinasi Ekstrak Daun Sirih Dan Belimbing Wuluh Dengan Metode Hidroekstraksi. Chempublish J. 2021;6(2):63–74.
25. Wijaya I. Potensi Daun Alpukat Sebagai Antibakteri. Jurnal Ilmu Kesehatan Sandi Husada. 2020;12(2):695–701. https://doi.org/10.35816/jiskh.v12i2.381
26. Faradina AS, Mastra N, Karta IW. Uji Aktivitas Antibakteri Ekstrak Etanol Akar Encok (Plumbago zeylanica L.) Terhadap Pertumbuhan Pseudomonas aeruginosa Secar In Vitro. Meditory. 2019;7(2):110–8.
27. Meilaningrum AN, Putri NEK, Sastyarina Y. Uji Aktivitas Antibakteri Ekstrak Kombinasi Umbi Bawang Tiwai dan Kulit Buah Lemon Terhadap Pertumbuhan Staphylococcus aureus dan Escherichia coli. Proceeding Mulawarmn Pharm Conf.2021;13(April 2021):8–13. https://doi.org/10.25026/mpc.v13i1.436
28. Daud NS, Arni DP, Idris SA, Saehu MS. Uji Aktivitas Antibakteri Ekstrak Batang Meistera chinensis Terhadap Escherichia coli ATCC 35218.War Farm. 2023;12(1):8–18. https://doi.org/10.46356/wfarmasi.v12i1.236
29. Lianah W, Ayuwardani N, Hariningsih Y. Aktivitas Antibakteri Ekstrak Etanol Seledri (Apium graveolens L) Terhadap Pertumbuhan Bakteri Actinomyces sp. dan Lactobacillus acidophilus. Duta Pharma J. 2021;1(1):32–9.
30. Sakul G, Simbala HEI, Rundengan G. Uji Daya Hambat Ekstrak Etanol Daun Pangi (Pangium edule Reinw. ex Blume) terhadap bakteri Staphylococcus aureus, Escherichia coli dan Pseudomonas aeruginosa. Pharmacon. 2020;9(2):275.
31. Wahyuni R, Guswandi, Rivai H. Pengaruh Cara Pengeringan Dengan Oven, Kering Angin dan Cahaya Matahari Langsung Terhadap Mutu Simplisia Herba Sambiloto. Fak Farm Univ Andalas Sekol Tinggi Ilmu Farm Padang. 2014;6(2):126–33.
32. Mulyono LM. Aktivitas antibakteri ekstrak etanol biji buah pepaya (Carica papaya L.) terhadap Escherichia Coli dan Staphylococcus aureus. Calyptra J Ilm Mhs Univ Surabaya [Internet]. 2013;2(2):1–9.
33. Busman B, Edrizal E, Utami DWP. Uji Efektivitas Ekstrak Buah Anggur Hijau (Vitis Vinivera L.) Terhadap Daya Hambat Laju Pertumbuhan Bakteri Streptococcus Mutans Dan Lactobacillus Acidophilus. Ensiklopedia Sos Rev. 2021;2(3):325–32.
34. Tortora G, Funke B, Case C. Microbiology : Make the Connection Between Lecture , Lab , and the Real World. 2013. Chapter 8.
35. Risna YK, Sri-Harimurti SH, Wihandoyo W, Widodo W. Kurva Pertumbuhan Isolat Bakteri Asam Laktat dari Saluran Pencernaan Itik Lokal Asal Aceh. J Peternak Indones (Indonesian J Anim Sci. 2022;24(1):1. https://doi.org/10.25077/jpi.24.1.1-7.2022
Published
2024-12-19
How to Cite
Fransiska, A., Fauziah, R. and Sari, D. (2024) “UJI AKTIVITAS ANTIBAKTERI EKSTRAK BUAH RAMBUSA (Passiflora foetida L.) TERHADAP PERTUMBUHAN BAKTERI Lactobacillus acidophilus ATCC 4356”, Jurnal Kesehatan Gigi dan Mulut (JKGM), 6(2), pp. 36-44. doi: 10.36086/jkgm.v6i2.2552.
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