IDENTIFIKASI METALLO-β-LACTAMASE (MBL) PADA ISOLAT BAKTERI GRAM NEGATIF DI RUMAH SAKIT KOTA PALEMBANG

Authors

DOI:

https://doi.org/10.36086/jpp.v18i1.1424

Keywords:

Fenotif, Genotif, Gram negatif, MBL

Abstract

Latar Belakang: Resistensi antibiotik merupakan ancaman bagi efektifitas pencegahan dan pengobatan terhadap penyakit infeksi. Antibiotik beta laktam ditemukan pertama kali sampai sekarang, angka kejadian infeksi oleh bakteri penghasil ESBL semakin meningkat diseluruh dunia. Ada 3 kelas menurut klasifikasi Ambler, diantara nya kelas B yaitu metallo-beta-lactamase (MBL) paling banyak menyebar dan banyak didominasi oleh bakteri Enterobactericeae yang merupakan bagian dari bakteri gram negatif. mengetahui prevalensi enzim Metallo beta lactamase (MBL) pada isolat bakteri gram negatif berdasarkan metode fenotif dan genotif sehingga akan didapatkan data terkait resistensi antibiotik ESBL khususnya Metallo-ꞵ-Lactamase. Metode: Penelitian ini berupa studi laboratorik dengan survei klinis secara cross sectional. Konfirmasi fenotif menggunakan Double-disk synergy test (DDST) dan Combined disk synergy test (CDST) dan secara genotif dengan mendeteksi gen IMP, VIM dan NDM. Hasil: Total 88 isolat didapatkan hasil uji DDST positif sebanyak 44(50%) isolat dan CDST sebanyak 87(98.8%) isolat. Konfirmasi MBL secara genotif didapatkan positif hanya pada gen IMP 7(7.9%) isolat, sedangkan pada gen VIM dan NDM semuanya negatif. Data secara fenotif pada sampel yang positif gen IMP, didapatkan sebagai berikut yaitu DDST positif sebanyak 4(4.5%) dan CDST positif sebanyak 7(7.9%). Sensitifitas dari perbandingan dua metode tersebut diperoleh hasil sebanyak 51.1% dan Spesifisitas sebanyak 100%. bakteri terbanyak yaitu K.pneumonia 45(51,1%) dan E.coli 31(35,2%). Sampel terbanyak sputum 34(38,6%); pus 18(20,5%) serta urine 13(14,8%).

Kesimpulan: Metode konfirmasi secara fenotif yang lebih mudah dipakai yaitu menggunakan metode CDST dan masih terdapat gen positif MBL pada isolate gram negatif.

 

Kata kunci : Fenotif, Genotif, Gram negatif, MBL

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Published

2023-06-30

Issue

Vol. 18 No. 1 (2023): JPP (Jurnal Kesehatan Poltekkes Palembang)

Section

Articles

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